Simple and reliable enumeration of Escherichia coli concentrations in wastewater samples by measuring β-d-glucuronidase (GUS) activities via a microplate reader

•    E. coli enumeration methods require high cost or skilled technique.

•    E. coli could be enumerated only by measuring intrinsic GUS activity in wastewater.

•    No pretreatment was required, and the cost was 0.02 USD per sample.

•    E. coli could be monitored over 10 months and at different treatment stages.

•    The wastewater matrix did not inhibit the measurement.

Credit: iStock, rusm

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