Objectives:Recently, many epidemiologic and animal studies have indicated that obesity has its origin in early stages of life, including the inappropriate balance of some nutrients. So the objectives of this study were to determine the risk of obesity in male offspring mice as a consequence of maternal vitamin D (VD) deficiency mediating the disordered immune response. Methods:C57BL/6J female mice 4 wk old were fed VD-deficient or normal reproductive diets during pregnancy and lactation. Their male offspring were given control and high-fat diets for 16 wk after weaning and then weighed and euthanized. The serum was collected for biochemical analyses. Epididymal (eWAT) and inguinal white adipose tissue (iWAT) were excised for histologic examination, immunohistochemistry, gene expression of inflammatory factors, and determination by flow cytometry of the proportions of immune cells.

 

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Citation: Li P, Li P, Liu Y, Liu W, Zha L, Chen X, Zheng R, Qi K, Zhang Y. Maternal vitamin D deficiency increases the risk of obesity in male offspring mice by affecting the immune response. Nutrition. 2021 Feb 11;87-88:111191. doi: 10.1016/j.nut.2021.111191. Epub ahead of print. PMID: 33744641.