Abstract As humans become increasingly urban and spend more time inside the built environment, there will be increased interactions between humans and shared public surface microbiomes. Recent cultural changes in the United States have led to increased numbers of gender‐neutral bathrooms. Given that bathroom surfaces are frequently sanitized, we used this increased availability of gender‐neutral bathrooms to examine how single‐gender or gender‐neutral surfaces are recolonized with microbes. Given that male and female microbiomes vary, we hypothesized that rates of recolonization would differ between male, female and gender‐neutral bathroom surfaces.

 

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