Risk factors for human acute leptospirosis in northern Tanzania

Abstract

Introduction

Leptospirosis is a major cause of febrile illness in Africa but little is known about risk factors for human infection. We conducted a cross-sectional study to investigate risk factors for acute leptospirosis and Leptospira seropositivity among patients with fever attending referral hospitals in northern Tanzania.

Methods

We enrolled patients with fever from two referral hospitals in Moshi, Tanzania, 2012–2014, and performed Leptospira microscopic agglutination testing on acute and convalescent serum. Cases of acute leptospirosis were participants with a four-fold rise in antibody titers, or a single reciprocal titer ≥800. Seropositive participants required a single titer ≥100, and controls had titers <100 in both acute and convalescent samples. We administered a questionnaire to assess risk behaviors over the preceding 30 days. We created cumulative scales of exposure to livestock urine, rodents, and surface water, and calculated odds ratios (OR) for individual behaviors and for cumulative exposure variables.

Results

We identified 24 acute cases, 252 seropositive participants, and 592 controls. Rice farming (OR 14.6), cleaning cattle waste (OR 4.3), feeding cattle (OR 3.9), farm work (OR 3.3), and an increasing cattle urine exposure score (OR 1.2 per point) were associated with acute leptospirosis.

Conclusions

In our population, exposure to cattle and rice farming were risk factors for acute leptospirosis. Although further data is needed, these results suggest that cattle may be an important source of human leptospirosis. Further investigation is needed to explore the potential for control of livestock Leptospira infection to reduce human disease.

Author summary

Leptospirosis is an under-recognized but important cause of febrile illness and death in Africa. The bacteria that cause leptospirosis have their usual life cycle in animals; humans are infected as accidental hosts. There is considerable variation between countries as to which reservoir animals and human activities are important for transmission of leptospirosis to humans. In many tropical countries flooding and rodents are the dominant sources of human infection. However, in Africa it is unknown which sources of leptospirosis are most responsible for human infection and what behaviors put people at risk for infection We performed a prospective cross-sectional study, to identify risk factors for acute leptospirosis and sources of human infection. We identified contact with cattle and work in rice fields as risk factors for acute leptospirosis. Our findings indicate that cattle may be an important source for human leptospirosis, and therefore control of leptospirosis in livestock may help prevent leptospirosis in people. Further work is needed to isolate Leptospira from humans and livestock. Rice farming was an uncommon activity in our study, but strongly associated with acute leptospirosis. Efforts are warranted to prevent infection in rice farmers living in Africa.

 

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By | 2018-06-09T12:37:20+00:00 June 9th, 2018|

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